serum no level  (Elabscience Biotechnology)

Journal: Scientific Reports

Article Title: DDR2 ameliorates nonalcoholic hepatic steatosis by activating the AMPK/ACC pathway

doi: 10.1038/s41598-026-42992-0

Figure Lengend Snippet: DDR2 overexpression in hepatocytes mitigates gluconeogenesis and lipid deposition in db/db mice. ( A ) Pattern diagram of treated mice ( n = 6). ( B–C ) DDR2 mRNA ( B ) and protein ( C ) levels in the livers of db/db mice, 11 days following infection with Ad-GFP or Ad-DDR2 ( n = 6). ( D ) Representative electron microscopy images of liver sections, scale bar 1 μm; H&E-stained sections, scale bar 50 μm; Oil Red O-stained sections, scale bar 50 μm and F4/80 staining, scale bar 30 μm. ( E ) Serum glucose concentrations in 6-hour-fasted db/db mice. ( F ) Reduced serum TG induced by DDR2 overexpression. ( G ) GTT performed in Ad-GFP- or Ad-DDR2-injected db/db mice, 9 days post-injection. ( H ) PEPCK and Pgc-1α protein level in liver tissues from the two groups. ( I ) There was no significant change in TG content in the liver. ( J ) AMPK and ACC phosphorylation and Srebp-1c, Fasn and Scd-1 protein levels in liver tissue. ( K ) Reduced serum TNF-α contents following DDR2 overexpression. ( L ) eIF-2α and IRE-1α phosphorylation in mouse livers. Data are shown as mean ± SEM, with comparisons by univariate t-tests. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Serum TNF-α levels were ascertained through an ELISA kit (E-MSEL-M0002, Elabscience Biotechnology Inc., Wuhan, China).

Techniques: Over Expression, Infection, Electron Microscopy, Staining, Injection, Phospho-proteomics

Journal: Frontiers in Immunology

Article Title: Macrophage-derived CCL20 promotes abdominal aortic aneurysm progression via lymphocytes CCR6

doi: 10.3389/fimmu.2026.1780720

Figure Lengend Snippet: Macrophages Exhibit Altered Gene Expression and Secrete Abundant CCL20. (A) The volcano plot of DEGs of macrophages in the normal and AAA groups in GSE166676 and GSE226492 . (B) Bubble plot of GO BP enrichment results for DEGs. (C) Bubble plot of enriched KEGG pathways for DEGs. (D) GSEA results based on KEGG identified activated and suppressed pathways of macrophages in the normal and AAA groups. Categories with red dots (NES > 0) showed the activated pathways in AAA group, while categories with blue dots (NES < 0) showed the activated pathways in the normal group. (E) The mRNA expression of IL-1beta, IL-8, TNF-α in normal and AAA tissues from human. (F) The mRNA expression of ACC1, FASN, and PPAR-γ in human Normal and AAA groups. (G) Representative images of Oil red O staining of the normal (n=5) and AAA group (n=6). Scale bar:10, 25μm. (H) All up DEGs in the Cytokine-Cytokine receptor interaction pathway. (I) CCL20 expression in 11 cell types. (J) Representative images of IF co-localization of CCL20 (green) and macrophages (CD68, red). Nuclei were stained with DAPI (blue), scale bar, 50 μm. The synchronized fluctuations of CCL20 (green) and macrophages (CD68, red) curves indicated co-localization at these discrete sites. (K) CCL20 expression in 11 cell types between the normal group and AAA groups. (L) ELISA test of CCL20 in the normal group (n=79) and AAA group (n=80). (M) ROC curve comparing models for AAA diagnosis. The model achieved an area under the curve (AUC) of 0.7. (N) Representative images of IF of CCL20 (green) expression in the the normal (n=5) and AAA group (n=6). Nuclei were stained with DAPI (blue), scale bar, 50 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Serum CCL20 levels in AAA patients and healthy controls were quantified using a commercial ELISA kit (Proteintech, KE00149, China), following the manufacturer’s protocol.

Techniques: Gene Expression, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Journal: Frontiers in Immunology

Article Title: Macrophage-derived CCL20 promotes abdominal aortic aneurysm progression via lymphocytes CCR6

doi: 10.3389/fimmu.2026.1780720

Figure Lengend Snippet: Macrophages Recruit Significant Numbers of Immune Cells through the CCL20-CCR6 Axis. (A) CCR6 expression in 11 cell types in GSE166676 and GSE226492 . (B) Representative images of IF co-localization of CCR6 (green) and B cells (CD19, red). Nuclei were stained with DAPI (blue), scale bar, 50μm. (C) Representative images of IF co-localization of CCR6 (green) and T cells (CD3, yellow). Nuclei were stained with DAPI (blue), scale bar, 50μm. The synchronized fluctuations of CCR6 (green)and T cells (CD3, yellow) curves indicated co-localization at these discrete sites. (D) CCR6 expression in 11 cell types. (E) CCR6 protein expression in human tissues between the normal group and the AAA group. (F, G) Representative images of IF of CCR6 (green) expression in the the normal (n=5) and AAA group (n=6). Nuclei were stained with DAPI (blue), scale bar, 50 μm. (H) CCR6 mRNA expression in GSE269845 and GSE183464 . (I) Cell Chat between macrophages and other cells in the CCL pathway signaling. (J) Representative images of IF of Macrophages (CD68, green), B cells (CD19, red), T cells (CD3, yellow) in the normal and AAA groups. Nuclei were stained with DAPI (blue), scale bar, 50 μm. (K) Flow cytometry of CD19 cells frequency in the LPS (100ng/ml) group and the LPS + CCL20 Antibody group (L) Flow cytometry of CD3 cells frequency in LPS (100ng/ml) group and the LPS + CCL20 Antibody group, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Serum CCL20 levels in AAA patients and healthy controls were quantified using a commercial ELISA kit (Proteintech, KE00149, China), following the manufacturer’s protocol.

Techniques: Expressing, Staining, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Macrophage-derived CCL20 promotes abdominal aortic aneurysm progression via lymphocytes CCR6

doi: 10.3389/fimmu.2026.1780720

Figure Lengend Snippet: Targeting the CCL20-CCR6 Axis Suppresses AAA Progression. (A) Schematic representation of study design, n=15 per group. (B) RT-qPCR of knockdown efficiencies for CCL20 and CCR6. (C) The AAA incidence. (D) The maximal external aortic diameter (mm). (E) Representative images of morphology of the whole aorta of all groups. (F) Representative vascular Doppler ultrasound images of the aorta of all groups. (N = 15). (G) Representative hematoxylin and eosin (HE), Elastica van Gieson (EVG), and elastin degradation grading, Masson trichrome staining. (H) Representative IF images of CCR6 (green), T cells (CD3, yellow), and B cells (CD19, red) in the aorta tissues of all groups. Nuclei were stained with DAPI (blue), scale bar, 20 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Serum CCL20 levels in AAA patients and healthy controls were quantified using a commercial ELISA kit (Proteintech, KE00149, China), following the manufacturer’s protocol.

Techniques: Quantitative RT-PCR, Knockdown, Staining

Journal: Frontiers in Immunology

Article Title: Macrophage-derived CCL20 promotes abdominal aortic aneurysm progression via lymphocytes CCR6

doi: 10.3389/fimmu.2026.1780720

Figure Lengend Snippet: The role of CCL20-CCR6 axis in AAA formation. Macrophage polarization was imbalanced, with enriched M1-like macrophages and elevated CCL20 secretion. CCL20 promoted the recruitment of CCR6+ immune cells (T and B cells) and AAA formation.

Article Snippet: Serum CCL20 levels in AAA patients and healthy controls were quantified using a commercial ELISA kit (Proteintech, KE00149, China), following the manufacturer’s protocol.

Techniques: